The Polarity Identification of Secondary Metabolite Compounds from Ethanol Extracts of Akar Bulu (Merremia fitovilia) Leaf through Thin Layer Chromatography (TLC) Analysis
This study aims to determine the solubility of secondary metabolites from ethanol extract of akar bulu (Merremiafitovilia) leaf. The research method through the stages of sample preparation in the form of sample collection, drying, and sieving; extraction using ethanol solvent and qualitative testing of solubility of secondary metabolites using thin layer chromatography (TLC). The results showed that the secondary metabolites from ethanol extract of Merremiafitovilia leaf were notinsoluble in n-hexane solvents, dissolved in chloroform and ethyl acetate solvents.Separation of secondary metabolites is most effective by using chloroform solvents.
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