Bacterial Cellulose Production from Combination of Coconut Water and Sago Wastewater
Abstract
Bacterial cellulose is biopolymer produced by microorganism, particularly bacteria of the genus Acetobacter. This research aims to determine (1) the effect of the combination of coconut water and sago wastewater as substrate for bacterial inoculum, and (2) the effect of the combination of coconut water and sago wastewater for bacterial cellulose production. This research was conducted with Completely Randomized Design (CRD) consisting of five treatments: SK1 (100% coconut water), SK2 (75% coconut water + 25% sago wastewater), SK3 (50% coconut water + 50% sago wastewater), SK4 (25% coconut water + 75% sago wastewater), and SK5 (100% sago wastewater). Each treatment consisted of 3 replications. The parameters measured were optical density of inoculum culture, fresh and dry weight of bacterial cellulose, and the thickness of bacterial cellulose. The results showed that the combination of coconut water and sago wastewater as substrate had an effect on inoculum quality and bacterial cellulose production. Treatment SK3 with a combination of 50% coconut water and 50% sago wastewater was the best substrate for bacterial inoculum with optical density value 0.59. The same medium was the best for bacterial cellulose production based on the thickness (1.02 cm) and weight of bacterial cellulose (443.33 g for fresh weight).
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Copyright (c) 2019 Pauline Destinugrainy Kasi, Eka Pratiwi Tenriawaru, Rosita Ridwan
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